Ever since Kary Mullis (that crazy guy, may he rest in peace) officially invented the polymerase chain reaction (PCR), an entire generation of molecular biology has exploded across the globe as scientists use PCR for a number of applications, from measuring gene expression to forensics. While the textbook technique is relatively simple, as I (and many other fellow researchers) can attest to from experience, producing an ideal PCR is far more challenging due to multiple factors.

When it comes to running a successful ELISA, there can be many common issues that must be addressed in order to obtain meaningful results. From our years of experience in producing and supplying ELISAs for a diverse range of targets, we’ve narrowed down four of the most common issues that can arise during your ELISA and have provided troubleshooting tips for each.