Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Publications (11) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Western blot analysis of various lysates using Acetyl-Histone H4-K8 Rabbit pAb (A7258) at 1:1000 dilution.NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Immunohistochemistry analysis of Acetyl-Histone H4-K8 in paraffin-embedded human kidney using Acetyl-Histone H4-K8 Rabbit pAb (A7258) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Immunohistochemistry analysis of Acetyl-Histone H4-K8 in paraffin-embedded mouse liver using Acetyl-Histone H4-K8 Rabbit pAb (A7258) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Immunohistochemistry analysis of Acetyl-Histone H4-K8 in paraffin-embedded rat kidney using Acetyl-Histone H4-K8 Rabbit pAb (A7258) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K8 Rabbit pAb (A7258). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K8 antibody (A7258) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

You may also interested in:

Overview

Product name Acetyl-Histone H4-K8 Rabbit pAb
Catalog No. A7258
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Immunogen information

Immunogen A synthetic acetylated peptide around K8 of human Histone H4 (NP_003529.1).
Sequence GGKGL
Gene ID 8359
Swiss prot P62805
Synonyms H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FA; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; HIST1H4A; Acetyl-Histone H4-K8
Calculated MW 11kDa
Observed MW 11kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:500 - 1:1000
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
  • ChIP-seq 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples 293T, NIH/3T3, C6
Cellular location Chromosome, Nucleus
Customer validation

WB (Mus musculus, Homo sapiens, Frog, Monascus ruber, Saccharomyces cerevisiae)

ChIP (Gallus gallus)

IF (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Acetyl-Histone H4-K8 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H4-K8 Rabbit pAb (A7258)}

Western blot - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Western blot analysis of various lysates using Acetyl-Histone H4-K8 Rabbit pAb (A7258) at 1:1000 dilution.NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Acetyl-Histone H4-K8 Rabbit pAb (A7258)}

Immunohistochemistry - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Immunohistochemistry analysis of Acetyl-Histone H4-K8 in paraffin-embedded human kidney using Acetyl-Histone H4-K8 Rabbit pAb (A7258) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H4-K8 Rabbit pAb (A7258)}

Immunohistochemistry - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Immunohistochemistry analysis of Acetyl-Histone H4-K8 in paraffin-embedded mouse liver using Acetyl-Histone H4-K8 Rabbit pAb (A7258) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H4-K8 Rabbit pAb (A7258)}

Immunohistochemistry - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Immunohistochemistry analysis of Acetyl-Histone H4-K8 in paraffin-embedded rat kidney using Acetyl-Histone H4-K8 Rabbit pAb (A7258) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Acetyl-Histone H4-K8 Rabbit pAb (A7258)}

Immunofluorescence - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K8 Rabbit pAb (A7258). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H4-K8 Rabbit pAb (A7258)}

Chromatin Immunoprecipitation - Acetyl-Histone H4-K8 Rabbit pAb (A7258)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K8 antibody (A7258) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Inquire About This Product

Submit your question about A7258 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on H4C1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H4C1. (Distance between topics and target gene indicate popularity.) H4C1

* Data provided by citexs.com, for reference only.

Publishing research using A7258? Please let us know so that we can cite the reference in this datasheet.

Antibodies (33)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order