Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-ATM-S1981 Rabbit pAb (AP0008)

Publications (10) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-ATM-S1981 Rabbit pAb (AP0008)

Western blot analysis of various lysates using (AP0008) at 1:1000 dilution.293T cells were treated by Doxorubicin (0.5 uM) at 37℃ for 24 hours.293T cells were treated by UV at room temperature for 15-30 minutes.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Overview

Product name Phospho-ATM-S1981 Rabbit pAb
Catalog No. AP0008
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S1981 of human ATM (NP_000042.3).
Sequence EGSQS
Gene ID 472
Swiss prot Q13315
Synonyms AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1; Phospho-ATM-S1981
Calculated MW 351kDa
Observed MW 370kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T, NIH/3T3
Cellular location Cytoplasmic vesicle, Nucleus
Customer validation

WB (Homo sapiens, Mus musculus)

IF (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-ATM-S1981 Rabbit pAb images

ABclonal:Western blot - Phospho-ATM-S1981 Rabbit pAb (AP0008)}

Western blot - Phospho-ATM-S1981 Rabbit pAb (AP0008)

Western blot analysis of various lysates using (AP0008) at 1:1000 dilution.293T cells were treated by Doxorubicin (0.5 uM) at 37℃ for 24 hours.293T cells were treated by UV at room temperature for 15-30 minutes.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ATM. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ATM. (Distance between topics and target gene indicate popularity.) ATM

* Data provided by citexs.com, for reference only.

Publishing research using AP0008? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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