Product Type > Antibodies > Antibody Duos > Phosphorylated Antibody Duos

Phospho-CREB1-S133 Antibody kit (RK04087)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-CREB1-S133 Rabbit pAb (AP0019)
ABclonal:Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0019)
ABclonal:Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0019)
ABclonal:Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0019)
ABclonal:Immunofluorescence - Phospho-CREB1-S133 Rabbit pAb (AP0019)
ABclonal:Western blot - CREB1 Rabbit pAb (A11989)
ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989)
ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989)
ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989)
ABclonal:Immunofluorescence - CREB1 Rabbit pAb (A11989)
ABclonal:Immunoprecipitation - CREB1 Rabbit pAb (A11989)

Overview

Product name Phospho-CREB1-S133 Antibody kit
Catalog No. RK04087

Product Component

Product name Catalog No. Positive Applications Species Reactivity
Phospho-CREB1-S133 Rabbit pAb AP0019 WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition Human, Mouse, Rat
CREB1 Rabbit pAb A11989 WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition Human, Mouse, Rat
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human CREB1 (NP_604391.1).
A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1).
Sequence MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD
RPSYR
Gene ID 1385
Swiss prot P16220
Synonyms CREB; CREB-1; Phospho-CREB1-S133
Calculated MW 35kDa
Observed MW 43kDa
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Cellular location Nucleus
Phospho-CREB1-S133 Rabbit pAb (AP0019)
ABclonal:Western blot - Phospho-CREB1-S133 Rabbit pAb (AP0019)}
Western blot - Phospho-CREB1-S133 Rabbit pAb (AP0019)

Western blot analysis of extracts of HeLa cells, using Phospho-CREB1-S133 pAb (AP0019) at 1:2000 dilution or CREB1 antibody (A11064).HeLa cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0019)}
Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0019)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Phospho-CREB1-S133 Rabbit pAb (AP0019) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0019)}
Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0019)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Phospho-CREB1-S133 Rabbit pAb (AP0019) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0019)}
Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0019)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-CREB1-S133 Rabbit pAb (AP0019) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-CREB1-S133 Rabbit pAb (AP0019)}
Immunofluorescence - Phospho-CREB1-S133 Rabbit pAb (AP0019)

Immunofluorescence analysis of MCF-7 cells using Phospho-CREB1-S133 antibody (AP0019). Blue: DAPI for nuclear staining.


CREB1 Rabbit pAb (A11989)
ABclonal:Western blot - CREB1 Rabbit pAb (A11989)}
Western blot - CREB1 Rabbit pAb (A11989)

Western blot analysis of various lysates using CREB1 Rabbit pAb (A11989) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989)}
Immunohistochemistry - CREB1 Rabbit pAb (A11989)

Immunohistochemistry analysis of CREB1 in paraffin-embedded human esophageal cancer using CREB1 Rabbit pAb (A11989) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989)}
Immunohistochemistry - CREB1 Rabbit pAb (A11989)

Immunohistochemistry analysis of CREB1 in paraffin-embedded mouse brain using CREB1 Rabbit pAb (A11989) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CREB1 Rabbit pAb (A11989)}
Immunohistochemistry - CREB1 Rabbit pAb (A11989)

Immunohistochemistry analysis of CREB1 in paraffin-embedded rat lung using CREB1 Rabbit pAb (A11989) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - CREB1 Rabbit pAb (A11989)}
Immunofluorescence - CREB1 Rabbit pAb (A11989)

Immunofluorescence analysis of HeLa cells using CREB1 Rabbit pAb(A11989) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CREB1 Rabbit pAb (A11989)}
Immunoprecipitation - CREB1 Rabbit pAb (A11989)

Immunoprecipitation analysis of 300 μg extracts of LO2 cells using 3 μg CREB1 antibody (A11989). Western blot was performed from the immunoprecipitate using CREB1 antibody (A11989) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on CREB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CREB1. (Distance between topics and target gene indicate popularity.) CREB1

* Data provided by citexs.com, for reference only.

Publishing research using RK04087? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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