The nucleosome consists of an octamer composed of four histones (H2A, H2B, H3, and H4) and a DNA entangled with 147 base pairs. The core of the histones constituting the nucleosome are roughly the same, but the free N-terminus can be subjected to various modifications.
The most studied histone modifications are methylation and acetylation, which generally enhance transcriptional activation by relaxing the histone interaction with genomic DNA. Histone methylation is a major determinant of the formation of active and inactive regions of the genome and plays a key role in the correct programming of the genome during development. Histone methylation of histones H3 (2, 17, 26) and H4 (position 3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs). Lysine methylation occurs mainly in histone H3 (4, 9, 27, 36, 79) and H4 (20) and has been implicated in transcriptional activation and silencing. Methylation of histones is separate from methylation of cytosine on the DNA helix, which has the effect of recruiting additional factors to further silence the surrounding target gene.
ABclonal offers diverse antibody products for the study of histone modifications and epigenetics to accommodate your research applications. Please feel free to contact us for more information, and happy experimenting!
The histone H3 antibody can be used as loading control for your histone modification research.