G1/S Cell Cycle Checkpoint Antibody

Panyue (Penny) Hao

Panyue (Penny) Hao
Mar 29, 2019 11:09:52 AM

The G1/S cell cycle checkpoints control whether eukaryotic cells enter the S phase (synthesis phase) of DNA synthesis after having properly completed the G1 phase to ensure the cell has enough energy and resources to begin DNA replication. Two cell cycle kinase complexes, CDK4/6-Cyclin D and CDK2- Cyclin E, work together to relieve the inhibition of dynamic transcriptional complexes containing retinoblastoma protein (Rb) and E2F. In cells undefined during the G1 phase, hypophosphorylated Rb binds to the E2F-DP1 transcription factor and forms an inhibitory complex with HDAC, thereby inhibiting downstream key transcriptional activities. Clear entry into the S phase is achieved by continuous phosphorylation of Rb by Cyclin D-CDK4/6 and Cyclin E-CDK2, which separates the transcription factor E2F from the inhibitory complex and allows transcription of the gene required for DNA replication. After the growth factor disappears, the expression level of cyclin D is down-regulated by down-regulation of protein expression and phosphorylation-dependent degradation. Without a proper G1/S checkpoint, the cell could arrest or potentially undergo aberrant processes that could lead to disease states such as cancer.


KO-validated CDK6 Polyclonal Antibody, Cyclin D1 Polyclonal Antibody, KO-validated CCND3 Polyclonal Antibody


With ABclonal's KO-validated antibodies, you can be assured that the antibody is specific against your desired target, and the band you see is in fact the band you are looking for!

CDK6 WB          Cyclin D1 WB          CCND3 WB

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Phospho-Rb-S811 Polyclonal Antibody, DP1 Polyclonal Antibody

ABclonal has antibodies that target specific post-translational modifications including phosphorylation at specific serine, threonine, and tyrosine residues, as well as other common and unique modifications such as acetylation and methylation!

Phospho Rb Immunofluorescence          DP1 immunohistochemistry

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Tags: Antibodies, Knockout Validated, Featured Product Weekly, Transcription, DNA Synthesis

Panyue (Penny) Hao

Panyue (Penny) Hao

Content creator, knowledge craver, all-around food fanatic thriving to survive in the fascinating yet uncharted world of biology.