4 Reasons to Use ABscript II RT-qPCR Kit

Lingyi Tong

Lingyi Tong
Mar 31, 2020 5:21:21 PM

The current COVID-19 public health crisis is unprecedented in the U.S. and worldwide. Everyone, including the U.S. Food and Drug Administration, medical workers, and biology researchers are busy doing whatever they can to fight against this epidemic. To help them handle the emergency and buy time for the suffering patients, ABclonal developed ABScript II One Step RT-qPCR Probe Kit (RK20407), a ready-to-use kit that can be used to quantify RNA with outstanding performance. Here, we would like to introduce the kit as well as the four reasons that you should use it.

ABScript II One Step RT-qPCR Probe Kit a ready-to-use kit allowing reverse transcription and subsequent probe-based qPCR in a single tube. It contains all components for RT-qPCR except for primers, probes and RNA templates, which means it can be used to quantify any RNA target. For a COVID-19-specific RT-qPCR kit, check out 3DMed's SARS-CoV-2 diagnostic kit, which have a limit of detection of 5 copies per reaction.

Product components (can be purchased separately)

2X One Step RT-qPCR Probe Buffer RM21462
One Step Probe HS Taq RM21463
ABScript II RT Enzyme Mix RM21464
50X ROX Dye I RM21465
50X ROX Dye II RM21466
Nuclease-free H2O RM20214

ABScript II One Step RT-qPCR Probe KitAnd here are the four reasons that you should use it.

1. All in one tube

This One Step Kit allows reverse transcription and subsequent probe-based qPCR to be completed in a single tube. After reverse transcription, the hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the subsequent PCR reaction, the 5 '-3 'exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. The one-step format significantly improves sensitivity and effectively prevent contamination

2. High sensitivity

The following figures demonstrate the extraordinary sensitivity of ABScript II One Step RT-qPCR Probe Kit.

SARS-CoV-2 well detected in range of 102-105 copies

Using pseudovirus synthesized from SARS-CoV-2 viral sequence as the template, diluted into 105, 104, 103, 102 and 10 copies.

Higher amplification ability compared to other brand 

image-92Using rat RNA as template (150ng input), the 5' VIC fluorescently labeled probes were diluted 5 times, each time by a factor of 10.

 

3. Good stability

The kit shows great stability under different temperatures and multiple freeze-thaw cycles

Enzyme Stability Test(Store at 4℃ for 1/3/5/7 days vs Control)

 

Freeze-Thaw Test (10/30/50/70/100 Cycles vs Control)

4. Compatible with all instruments

Depend on the color dye you're using, the kit is compatible with the most common real-time PCR instruments in the market. Here's our recommendation list: 

50X ROX Reference Dye I
Applied Biosystems 7000/7300/7700/7900, Applied Biosystems StepOneTM/StepOnePlusTM
50X ROX Reference Dye II
Applied Biosystems 7500/ViiA7TM, QuantStudioTM , Stratagene Real-time PCR Systems, Rotor-geneTM 3000
NO ROX Reference Dye
Bio-Rad iCyclers/ CFX96/ CFX 384, Roche Light Cyclers® , QIAGEN/Corbett Systems, Eppendorf Mastercyclers®

 

If you have any questions about the product or want to order in bulk, please call us at 888-754-5670 or email us for detailed information.

 

Tags: Reverse Transcription, Real-time PCR, PCR, Molecular Biology

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