---
title: ABclonal Knowledge Base | Molecular Biology
description: Molecular Biology |
---
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[](https://blog.abclonal.com/blog/5-steps-to-a-better-pcr)
Jul 29, 2022, 12:00:00 PM by [Kin Leung](https://blog.abclonal.com/blog/author/kin-leung)
## [5 Steps to a Better PCR: A Troubleshooting and Optimization Guide](https://blog.abclonal.com/blog/5-steps-to-a-better-pcr)
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Ever since Kary Mullis (that crazy guy, may he rest in peace) officially [invented the polymerase chain reaction](https://www.nobelprize.org/prizes/chemistry/1993/summary/) (PCR), an entire generation of molecular biology has exploded across the globe as scientists use PCR for a number of applications, from measuring gene expression to forensics. While the textbook technique is relatively simple, as I (and many other fellow researchers) can attest to from experience, producing an ideal PCR is far more challenging due to multiple factors.
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---
[](https://blog.abclonal.com/blog/interleukin-family-overview)
Nov 19, 2021, 12:00:00 PM by [Eva Volckova](https://blog.abclonal.com/blog/author/eva-volckova)
## [Interleukin Family Overview](https://blog.abclonal.com/blog/interleukin-family-overview)
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Interleukins are a group of small signaling molecules, and a type of cytokine. They play a vital role in the body’s immune response by activating and deactivating immune cells. Recently, interleukins have gained visibility as a target to help treat COVID-19, and the WHO has recommended giving IL-6 inhibitors to patients with severe cases. Additionally, because of its widespread impact on the body, the interleukin family has gained popularity as drug targets over the last few years.
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---
[](https://blog.abclonal.com/blog/dna-damage-repair-and-its-therapeutic-potential)
Oct 29, 2021, 10:30:00 AM by [Eva Volckova](https://blog.abclonal.com/blog/author/eva-volckova)
## [DNA Damage & Repair and its Therapeutic Potential](https://blog.abclonal.com/blog/dna-damage-repair-and-its-therapeutic-potential)
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Since DNA was first discovered by researchers, decades of work have been done to understand its importance as it is the code of life itself. While DNA is the cornerstone of life, it is not immune to damages, and as so it is vital for DNA to repair itself for normal cell function to be maintained. Though, DNA is not always able to repair itself and this leads to some diseases such as various cancers. Fortunately, DNA repair pathways are capable of being tools to provide therapies to combat these diseases.
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---
[](https://blog.abclonal.com/blog/webinar-recap-mechanisms-for-transcriptional-regulation-of-myogenic-and-metal-homeostasis-genes)
Feb 1, 2021, 3:53:38 PM by [Dennis Miao](https://blog.abclonal.com/blog/author/dennis-miao)
## [Transcriptional Regulation of Myogenic and Metal Homeostasis Genes](https://blog.abclonal.com/blog/webinar-recap-mechanisms-for-transcriptional-regulation-of-myogenic-and-metal-homeostasis-genes)
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On January 12, 2021, we had the privilege of hosting Dr. Teresita Padilla-Benavides, an Assistant Professor of Molecular Biology and Biochemistry at Wesleyan University in Middletown, CT, to present our first webinar of the new year. Her webinar discussed her research on the differential mechanisms for transcriptional regulation of myogenic and metal homeostasis genes. If you missed the live session of the webinar, we’ve got you covered here with a [**link to a recording**](https://us02web.zoom.us/rec/share/OtNU4caeNbuqIbjjLaORZ72vNiE0LK8xl9PTEqkMZ2j9z3d_4-o2eg2xCVlE9lAP.IxaYIFxMTJ1qcK_e) of the webinar, as well as a recap below:
[Read More](https://blog.abclonal.com/blog/webinar-recap-mechanisms-for-transcriptional-regulation-of-myogenic-and-metal-homeostasis-genes)
---
[](https://blog.abclonal.com/blog/a-closer-look-at-the-fundamentals-of-recombinant-dna-technology)
Oct 27, 2020, 4:09:38 PM by [Bryent Lee](https://blog.abclonal.com/blog/author/bryent-lee)
## [A Closer Look at the Fundamentals of Recombinant DNA Technology](https://blog.abclonal.com/blog/a-closer-look-at-the-fundamentals-of-recombinant-dna-technology)
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The advancement of recombinant DNA technology in recent years has drastically changed the world of research by controlling the expressions of target genes. Recombinant DNA combines genetic material from different sources, creating sequences that are unique and new to the genome. The DNA sequences used in the construction of recombinant DNA molecules can originate from any species, such as human, fungal, bacterial, and plants. 1
[Read More](https://blog.abclonal.com/blog/a-closer-look-at-the-fundamentals-of-recombinant-dna-technology)
---
[](https://blog.abclonal.com/blog/breaking-the-bad-an-introduction-to-proteinase-k)
May 27, 2020, 3:44:20 PM by [Dennis Miao](https://blog.abclonal.com/blog/author/dennis-miao)
## [Breaking the Bad: An Introduction to Proteinase K](https://blog.abclonal.com/blog/breaking-the-bad-an-introduction-to-proteinase-k)
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As you may have surmised from the title of this article, Proteinase K (also known as *protease K* or *endopeptidase K*) shares many functional similarities to the protagonist of the iconic TV show, *Breaking Bad*. Much like Walter White, Proteinase K is incredibly versatile in its applications, while remaining relatively unassuming and overlooked at times. Unlike the chemistry teacher gone rogue, however, its properties can be channeled for good.
[Read More](https://blog.abclonal.com/blog/breaking-the-bad-an-introduction-to-proteinase-k)
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