Do you find yourself using a lot of SDS-PAGE gels for Western blotting and Coomassie staining? Or have your pre-cast gels been stored for too long and expired? Why not try to cast your own SDS-PAGE gels to save some budget for the lab, and produce just as valid of results. Today, we would like to share five tips for hand-casting SDS-PAGE gels, as well as the protocol and formulation to do so.