The arguably most fun thing about science is when your supervisor tells you to just do Experiment X to test hypothesis, but then they kind of forget to tell you how complicated the techniques are to perform that experiment, not to mention all the optimization you would need to do. I personally have never done a chromatin immunoprecipitation (ChIP), and since I wasn’t in genomics, the most sequencing I ever did was setting up quick reactions for the core facility to tell me that my gene constructs were correctly built. ChIP does sound rather simple when explained in class, but when you read up on the protocols,1 there are some limitations to what ChIP can do, especially given the large amount of starting material you need for the typical experiment. Luckily, in recent years, scientists have started to use an alternative technique called Cleavage Under Targets and Tagmentation, or CUT&Tag, which ABclonal is pleased to support through our antibody reagents.
I will admit that I am not a neuroscientist, having focused my research on immunology and cancer cell biology, but I’ve always been aware of Alzheimer’s Disease and the quest for better treatments and an eventual cure. It is because I am not a neuroscientist that I rely on the word of purported experts in the field who have dedicated their careers to finding these answers. There are various caveats like the level of journal the research is published in, the quality of the images (at least to the naked eye), the number of times the research is cited, and the known reputation of the authors, that help to determine the level of trust one can put into the finding. Yet, we find that some things still might slip through the cracks, and this reminds us that we need to scrutinize data more thoroughly to hold each other accountable and maintain trust in science.
It seems like decades since the SARS-CoV-2 pandemic shut down the world economically and socially, and even now we are not fully out of the woods. The COVID-19 coronavirus continues to persist, hovering on the cusp of becoming an endemic disease after having caused over one million deaths in the United States alone out of over six million deaths worldwide since the first reported cases in 2019. Although the various coronavirus vaccines have conferred some level of herd immunity across the globe, the danger of mutations causing variants that might escape vaccine protection is real, so continued vigilance and best practices are key to returning to normalcy. Perhaps our resolve as a global community and as a species will be tested in short order as the monkeypox outbreaks surge.
Throughout the COVID-19 pandemic, I have been washing my hands with vigilance to prevent the spread of germs. As a result, the skin on my hands have become calloused on some parts and mostly dry, with cuts and slight bleeding on occasion. I thought this was inconvenient, but when I learned about children with a rare genetic skin disease, I stopped feeling sorry for myself and dug a bit deeper into their plight. After all, my skin issues are just due to excessive hand washing (which everyone should be doing anyway!); these poor kids have to live with this painful disease, known as dystrophic epidermolysis bullosa, for their entire lives.
Cell proliferation assays have a wide range of applications in scientific research – from testing drug reagents to the effect of growth factors, from testing cytotoxicity to analyzing cell activity. So, what are cell proliferation assays? Cell proliferation assays typically detect changes in the number of cells in a division or changes in a cell population.
In a previous article, we explored the differences between rabbit and mouse antibodies as well as the biology behind rabbit antibody superiority. But after choosing the host, the type of technology used to produce the antibody is important too. Here, we explore some of the rabbit monoclonal antibody technologies available in the current market.