Having worked in a proteomics lab for my PhD dissertation, I had some familiarity with the tools and strategies used to study biology on a systems level. One of the concepts I was always interested in was the ability to just follow a protein's journey throughout the cell, from the time it is translated by the ribosome to its final destination either within an organelle or when it is secreted into the extracellular space. At the time I was finishing up, I wasn't sure that the technology was yet advanced enough to make that a reality, particularly if done within a single cell. But within the past few years, a new era of spatial proteomics has emerged to allow us to observe cell biology in a whole new light.