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Featured Product Weekly: AIFM1 Antibody

Posted by Panyue (Penny) Hao on Jul 31, 2018 12:58:57 PM

AIFM1, also known as Apoptosis Inducing Factor (AIF), is a widely expressed flavoprotein that plays an important role in caspase-independent apoptosis. AIF normally exists in the mitochondrial intermembrane space.

After apoptotic stimuli, the permeability of the mitochondrial outer membrane changes, and AIF is released from the mitochondria. AIF usually synthesizes a 67 kDa precursor protein, which is then transported into the mitochondria and hydrolyzed to remove the amino terminal mitochondrial localization signal into a mature 57 kDa protein. In many cells, the 67 kDa precursor protein is how AIF predominantly exists. Treatment of isolated and purified nuclei with recombinant AIF can lead to early apoptotic events such as chromatin condensation and large DNA fragment formation.

Recent studies have shown that AIF has dual functions: a pro-apoptotic activity that occurs in the nucleus, where it binds DNA; and an anti-apoptotic activity where it finds free radicals through oxdoreductase activities.

AIFM1 Antibody

ABclonal's AIFM1 antibody (A2568) is a knockout-validated antibody that can be used in WB, IHC, and IF.

AIFM1 A2568

Get KO-validated AIFM1 Antibody

 

Apart from the functions mentioned above, AIFM1 also acts as a marker of mitochondrial organelles. Other similar mitochondrial markers include mitochondrial fission protein (FIS1) and  mitochondrial membrane ATP synthase (ATP5A1)

FIS1 ATP5A1

                                        Get FIS1 Antibody                                             Get ATP5A1 Antibody

 

Tags: Apoptosis, Organelle Marker, Mitochondria, Knockout Validated