Although underappreciated, the Golgi apparatus is indispensable to normal cellular function by ensuring proteins are properly folded and sorted, and to direct diverse functions including autophagy. Disruptions to proper Golgi function can lead to many disease states, including diabetes, cancer, and neurodegenerative disorders such as Alzheimer's disease.
Part of the Golgi protein family, USO1 protein (also known as vesicle docking protein p115) is a peripheral membrane protein that can be used as a Golgi marker. It cycles between the cytoplasm and the Golgi apparatus during interphase. The position of the USO1 protein is regulated by phosphorylation -- dephosphorylated proteins bind to the Golgi membrane and dissociate from the membrane when phosphorylated. This regulated transportation plays an important role in protein localization, secretion, and signal transduction. USO1 protein acts as a vesicle anchor by interacting with the target membrane and keeping the vesicles close to the target membrane. In addition, the USO1 protein interacts with GOLGA2 (GM130) and Giantin to promote endoplasmic reticulum-Golgi transportation. A large part of Golgi-related research is in understanding how to maintain proper Golgi function to prevent and treat human diseases.
The USO1 polyclonal antibody (A16079) has been rigorously validated by ABclonal quality control using WB and IF.
ABclonal's GOLGA2 polyclonal antibody (A5344) can be used for WB, IF, and IP.
And if you're looking for other helpful reads regarding proteins, be sure to check out our other related blogs here.