Many of you are well on your way through graduate school, itching to earn that precious PhD, while some are just starting out, getting ready to take your first midterms while preparing to choose your first research rotations. Regardless of where you are in your career, or even if you've already earned that doctorate and are on your way to a postdoc and beyond, ABclonal's blog series has put together some articles that can help you get through the day. Whether it is experimental troubleshooting or just trying to get along with your lab mates and PhD supervisor, here is a collection of previous blogs that should be of use to you. 

Ever since Kary Mullis (that crazy guy, may he rest in peace) officially invented the polymerase chain reaction (PCR), an entire generation of molecular biology has exploded across the globe as scientists use PCR for a number of applications, from measuring gene expression to forensics. While the textbook technique is relatively simple, as I (and many other fellow researchers) can attest to from experience, producing an ideal PCR is far more challenging due to multiple factors.