1. Determine the mRNA capture type you need

The scientific community operates on a self-correcting model that relies on repetition and replication. However, according to a 2016 survey by Nature, more than 70% reported to have failed to replicate experiments from another scientist, more than 50% reported failure in replicating his/her own experiment. Out of the 1,576 scientists surveyed, 906 were from biology or medicine disciplines. 

CTCF (CCCTC Binding Factor) is a highly conserved transcription factor that regulates transcriptional activation, transcriptional repression, insulator function, and imprinted control regions (ICRs).

Therapies targeting the function of a small intestinal protein, SGLT1, might have the potential to treat diseases like obesity, diabetes, heart failure, and associated death—and we have next generation sequencing to thank.

CREB1 is a basic leucine zipper domain (bZIP) transcription factor that activates a target gene through a cAMP response element. As a key transcriptional regulator, CREB1 plays a role in a variety of cellular responses by mediating a number of physiological stimuli. CREB1 is expressed in many tissues and plays an especially important regulatory role in the nervous system by promoting neuronal survival, driving precursor proliferation, neurite outgrowth, neuronal differentiation and more. In addition, CREB1 signaling is involved in the learning and memory functions of many organisms. CREB1 is capable of selectively activating many downstream genes through interaction with multiple dimerization partners. Phosphorylation of CREB1 at the serine 133 site involves multiple signaling pathways, such as Erk, calcium flux (Ca²⁺), and stress signaling. Some of the kinases involved in CREB1 phosphorylation include p90RSK, MSK, CaMKIV, and MAPKAPK-2.

Announcements for this year’s Nobel winners started off with prizes in Physics, Chemistry, and Physiology or Medicine. While congratulations are in order for the newly minted laureates, a bit of controversy is also stirring.